Journal: Oncotarget

Article Title: Plasma glutamate carboxypeptidase is a negative regulator in liver cancer metastasis

doi: 10.18632/oncotarget.12967

Figure Lengend Snippet: A. Semi-quantitative RT-PCR analysis of DKK4 expression in SK-Hep1 cells treated with PGCP supernatant after PGCP knockdown. ACTB was used as an internal control (top). The signal intensity corresponding to DKK4 was quantified using the ImageJ sofware (bottom). B. Western blot analysis after the DKK4 addition to PGCP knockdown cells. Top, Western blot analysis after transfection with FALG-Mock or FLAG-DKK4, respectively. After 24 h, the culture media including overexpressed DKK4 recombinant proteins was concentrated using an Amicon column. The samples were immunoblotted with anti-DKK4 antibody. Bottom, SK-Hep1 cells were treated with siRNAs (siCont and siPGCP) for 48 h, and secreted DKK4 was added to the culture media. After 24 h, Western blot analysis was performed using the indicated antibodies. ACTB was used as an internal control. C. Detection of phospho-GSK3β (Ser9) and β-catenin after siDKK4 silencing in SK-Hep1 and SNU-387 cells. Western blot analysis was performed using the indicated antibodies. ACTB was used as an internal control. D. Detection of phospho-LRP6 by Western blotting. SK-Hep1 and SNU-387 cells treated with siCont or siPGCP were grown with or without serum. Whole cell lysates were analyzed by Western blotting using the indicated antibodies. ACTB was used as a loading control. E. T4 assay using a Human Free Thyroxine ELISA kit. SK-Hep1 cells treated with siCont or siPGCP were cultured for 48 h. The p value was calculated using Student's t-test (*p<0.05) (top). Quantitative RT-PCR analysis of DKK4 mRNA level after T4 treatment for 24 h. ACTB was used as a loading control. The signal intensity corresponding to DKK4 was quantified using the ImageJ software (bottom). F. Detection of phospho-GSK3β (Ser9) and β-catenin. Western blot analysis was performed after treatment with T4 (final 100 ng/ml) in siRNA-treated SK-Hep1 cells using the indicated antibodies. ACTB was used as an internal control (top). Cell migration assay after T4 treatment. Cells treated with siCont or siPGCP were incubated with T4 (final 100 ng/ml) for 12 h before cell migration assay. DMSO was used as a negative control (bottom).

Article Snippet: The concentration of T4 was measured by the Human Free Thyroxine (FT4) ELISA kit (CSB-E05078h, CUSABIO, Wuhan, China).

Techniques: Quantitative RT-PCR, Expressing, Knockdown, Control, Western Blot, Transfection, Recombinant, Enzyme-linked Immunosorbent Assay, Cell Culture, Software, Cell Migration Assay, Incubation, Negative Control

Journal: Nutrients

Article Title: A Preliminary Analysis of the Influence of Elderberry Juice Consumption on Thyroid Metabolism in Mice and Humans Fed High-Fat Diets

doi: 10.3390/nu17040612

Figure Lengend Snippet: Violin plots with boxplot overlays showing concentrations of thyroid hormones ( A ) T4 (ng/mL), ( B ) TSH (ng/mL), and ( C ) Tg (ng/mL). The results compare the effects of EBJ and PL treatments in humans. EBJ, elderberry juice treatment; PL, placebo treatment; T4, thyroxine; TSH, thyroid stimulating hormone; Tg, thyroglobulin. Pair-wise comparisons without a common letter are significantly different, p < 0.05.

Article Snippet: Thyroxine (T4) concentrations in serum samples were measured using a Mouse Thyroxine ELISA Kit (Novus Biologicals, Centennial, CO, USA, NBP2-60162), following the manufacturer’s instructions.

Techniques:

Journal: Nutrients

Article Title: A Preliminary Analysis of the Influence of Elderberry Juice Consumption on Thyroid Metabolism in Mice and Humans Fed High-Fat Diets

doi: 10.3390/nu17040612

Figure Lengend Snippet: Violin plots with boxplot overlays showing concentrations of thyroid hormones T4 (ng/mL). The results compare the effects of EBJHFD and HFD treatments in mice. EBJHFD, elderberry juice + high-fat diet; HFD, high-fat diet; T4, thyroxine.

Article Snippet: Thyroxine (T4) concentrations in serum samples were measured using a Mouse Thyroxine ELISA Kit (Novus Biologicals, Centennial, CO, USA, NBP2-60162), following the manufacturer’s instructions.

Techniques: